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- Primary Antibodies ›
- PP2A alpha Antibodies
Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
View all (26) PP2A alpha antibodies
Cite PP2A alpha Polyclonal Antibody
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PP2A alpha Antibody (PA5-28419) in WB
Western blot was performed using Anti-PP2A alpha Polyclonal Antibody (Product # PA5-28419) and 36 kDa band corresponding to PP2A alpha was observed across the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HL-60 (Lane 1), A-431 (Lane2), PC-3 (Lane 3), HEL 92.1.7 (Lane 4), K-562 (Lane 5), A549 (Lane 6), HeLa (Lane 7), tissue extracts (30 µg lysate) of Mouse Brain (Lane 8) and Mouse Kidney (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferre... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
PA5-28419
Applications
Tested Dilution
Publications
Western Blot(WB)
1:500-1:10,000
-
Immunohistochemistry (Paraffin) (IHC (P))
1:100-1:1,000
-
Immunocytochemistry(ICC/IF)
1:100-1:1,000
-
Immunoprecipitation(IP)
1:100-1:500
-
Product Specifications
Species Reactivity
Human,Mouse,Rat
Host/Isotype
Rabbit/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to a region within amino acids 247 and 259 of Human PP2A alpha
Conjugate
Unconjugated
Form
Liquid
Concentration
0.479 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545895
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLaS3, Molt-4, Raji, mouse brain.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. The PP2A family comprises subfamily members PP2A alpha and PP2A beta. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases:B55-GAMMA; IMYPNO; Methyl-PPP2CA; MGC33570; PP2A alpha; PP2A C terminal; PP2A L9; PP2A Leucine 9; PP2A-alpha; PR52; PR55G; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2a, catalytic subunit, alpha isoform; protein phosphatase-2A-alpha; Replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
UniProt ID:(Human) P67775, (Mouse) P63330, (Rat) P63331
Entrez Gene ID:(Human) 5515, (Mouse) 19052, (Rat) 24672
Molecular Function: 
protein phosphatase
Function(s)
nuclear-transcribed mRNA catabolic process, nonsense-mediated decayinactivation of MAPK activityregulation of DNA replicationregulation of transcription, DNA-templatedprotein dephosphorylationceramide metabolic processapoptotic processmitotic nuclear envelope reassemblymesoderm developmentRNA splicingresponse to organic substancenegative regulation of epithelial to mesenchymal transitionsecond-messenger-mediated signalingregulation of Wnt signaling pathwayregulation of cell adhesionnegative regulation of cell growthregulation of growthnegative regulation of tyrosine phosphorylation of Stat3 proteinregulation of cell differentiationmeiotic cell cyclepositive regulation of protein serine/threonine kinase activitynegative regulation of transcription from RNA polymerase II promoterregulation of protein phosphorylationnegative regulation of protein phosphorylationregulation of receptor activityregulation of protein autophosphorylationpositive regulation of protein phosphatase type 2A activitypositive regulation of protein dephosphorylationregulation of protein catabolic processnegative regulation of transcription factor import into nucleuspositive regulation of apoptotic processpositive regulation of cysteine-type endopeptidase activity involved in apoptotic processregulation of cell cycleprotein heterotrimerizationcellular response to glucose stimulusnegative regulation of calcium ion transmembrane transporter activitycardiac ventricle developmentheart developmentcellular response to insulin stimuluscellular response to calcium ioncellular response to cytokine stimuluscellular response to ethanolcellular response to follicle-stimulating hormone stimuluscellular response to steroid hormone stimulus
Process(es)
phosphoprotein phosphatase activityprotein bindingprotein C-terminus bindingmetal ion bindingGABA receptor bindingprotein serine/threonine phosphatase activityhydrolase activityenzyme bindingprotein kinase bindingprotein phosphatase bindingprotein domain specific bindingprotein complex bindingprotein kinase B bindingion channel bindingprotein dimerization activitytau protein bindingprotein phosphatase 2A bindingprotein antigen bindingprotein heterodimerization activity
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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