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- CD1d Antibodies
Invitrogen
View all (36) CD1d antibodies
Cite CD1d Monoclonal Antibody (1B1), Functional Grade, eBioscience™
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CD1d Antibody (16-0011-82) in Flow
Staining of BALB/c splenocytes with 1.0 µg of Rat IgG2b K Isotype Control Functional Grade Purified (Product # 16-4031-81) (open histogram), or 1.0 µg of Anti-Mouse CD1d Functional Grade Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85).Total viable cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
16-0011-82
Applications
Tested Dilution
Publications
Western Blot(WB)
-
View 1 publication1 publication
Flow Cytometry(Flow)
1 µg/test
View 7 publications7 publications
Neutralization(Neu)
-
View 2 publications2 publications
Functional Assay(FN)
Assay-Dependent
-
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat/ IgG2b, kappa
Recommended Isotype Control
Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™
Class
Monoclonal
Type
Antibody
Clone
1B1
Conjugate
Functional Grade
- Alexa Fluor 488
- APC
- Biotin
- Brilliant Violet 480
- Brilliant Violet 711
- eFluor 450
- PE
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Wet ice
RRID
AB_468838
Product Specific Information
Description: The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein with structural hom*ology to MHC class I molecules. While similar to MHC Class I, CD1d associates with beta2m, functionally CD1d is similar to MHC Class II. 1B1 detects CD1d at varying levels on mouse leukocytes. 1B1 detects beta2m associated CD1d.
Applications Reported: The 1B1 antibody has been reported for use in flow cytometric analysis. 1B1 has also been reported in in vivo and in vitro studies.
Applications Tested: The 1B1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
CD1d is a heavy chain associated with Beta 2 microglobulin on cortical thymocytes. Beta 2 microglobulin independent expression of CD1d has also been demonstrated on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family of major histocompatibility (MHC) like glycoproteins. The CD1d gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by the CD1d gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. Diseases associated with CD1D include Mycobacterium Malmoense and Autoimmune Disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases:Antigen-presenting glycoprotein CD1d1; CD1d.1; T-cell surface glycoprotein CD1d1
UniProt ID:(Mouse) P11609
Entrez Gene ID:(Mouse) 12479
Molecular Function: 
major histocompatibility complex protein
Function(s)
positive regulation of T cell mediated cytotoxicityimmune system processimmune responseantigen processing and presentationpositive regulation of T cell proliferationpositive regulation of macrophage activationpositive thymic T cell selectionpositive regulation of interferon-gamma biosynthetic processpositive regulation of interleukin-2 biosynthetic processinnate immune responsepositive regulation of interleukin-4 biosynthetic processantigen processing and presentation, endogenous lipid antigen via MHC class Ibantigen processing and presentation, exogenous lipid antigen via MHC class Ibregulation of immune responsepositive regulation of NK T cell activationpositive regulation of NK T cell differentiation
Process(es)
protein bindingbeta-2-microglobulin bindinglipid antigen bindingendogenous lipid antigen bindingexogenous lipid antigen bindinghistone bindingT cell receptor bindingcell adhesion molecule bindinglipopeptide binding
It has to be done as per old AB suggested Products section.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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