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Cite IFN gamma Recombinant Rabbit Monoclonal Antibody (003), FITC
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IFN gamma Antibody (MA5-52308) in Flow
Flow cytometry analysis of Human IFN-gamma expression on human peripheral blood lymphocytes. Samples were incubated with IFN gamma monoclonal antibody (Product # MA5-52308) and PE-conjugated anti-Human CD3. Human peripheral blood mononuclear cells were stimulated for 4-6 hours with PMA and Ionomycin in the presence of GolgiPlug. The dot plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
MA5-52308
Applications
Tested Dilution
Publications
Flow Cytometry(Flow)
5 µL per test
-
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Clone
003
Immunogen
Recombinant Human IFN gamma/IFNG Protein (Gln 24-Gln166)
Conjugate
FITC
- PerCP
Excitation/Emission Max
498/517 nmView spectra
Form
Liquid
Concentration
100 µg/mL
Purification
Protein A
Storage buffer
PBS with 0.05% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a hom*odimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma hom*odimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any hom*ology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence hom*ology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases:H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Immune interferon; Interferon gamma; Interferon y; Interferon γ; interferon, gamma; Interferonγ; M-IFN-g; R-IFN-g
UniProt ID:(Human) P01579
Entrez Gene ID:(Human) 3458
Molecular Function: 
cytokine interferon superfamily intercellular signal molecule
Function(s)
protein import into nucleus, translocationnegative regulation of transcription from RNA polymerase II promoterneutrophil apoptotic processregulation of the force of heart contractionadaptive immune responseCD8-positive, alpha-beta T cell differentiation involved in immune responseapoptotic processmovement of cell or subcellular componenthumoral immune responsecell cycle arrestcell surface receptor signaling pathwaypositive regulation of cell proliferationresponse to viruspositive regulation of autophagypositive regulation of gene expressionnegative regulation of gene expressionpositive regulation of epithelial cell migrationantigen processing and presentationneutrophil chemotaxisnegative regulation of epithelial cell differentiationendoplasmic reticulum unfolded protein responsenegative regulation of myelinationpositive regulation of synaptic transmission, cholinergicnegative regulation of interleukin-17 productionpositive regulation of interleukin-12 productionpositive regulation of interleukin-23 productionpositive regulation of tumor necrosis factor productionpositive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation involved in immune responsepositive regulation of peptidyl-serine phosphorylation of STAT proteinpositive regulation of smooth muscle cell apoptotic processpositive regulation of T cell proliferationresponse to drugpositive regulation of tyrosine phosphorylation of Stat1 proteindefense response to bacteriumdefense response to protozoannegative regulation of growth of symbiont in hostpositive regulation of chemokine biosynthetic processpositive regulation of interleukin-12 biosynthetic processpositive regulation of MHC class II biosynthetic processpositive regulation of interleukin-6 biosynthetic processpositive regulation of nitric oxide biosynthetic processpositive regulation of neuron differentiationpositive regulation of osteoclast differentiationpositive regulation of transcription from RNA polymerase II promoterpositive regulation of isotype switching to IgG isotypesnegative regulation of smooth muscle cell proliferationpositive regulation of interleukin-1 beta secretionregulation of insulin secretionT cell receptor signaling pathwaysensory perception of mechanical stimuluspositive regulation of membrane protein ectodomain proteolysisdefense response to viruspositive regulation of killing of cells of other organisminterferon-gamma-mediated signaling pathwayregulation of interferon-gamma-mediated signaling pathwaypositive regulation of fructose 1,6-bisphosphate 1-phosphatase activitypositive regulation of fructose 1,6-bisphosphate metabolic processpositive regulation of vitamin D biosynthetic processpositive regulation of calcidiol 1-monooxygenase activitycellular response to lipopolysaccharidecellular response to interleukin-18positive regulation of establishment of protein localization to plasma membraneextrinsic apoptotic signaling pathwayregulation of neuronal action potentialpositive regulation of exosomal secretionpositive regulation of tumor necrosis factor (ligand) superfamily member 11 productionregulation of hepatocyte proliferation
Process(es)
cytokine activityinterferon-gamma receptor binding
It has to be done as per old AB suggested Products section.
