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- CD235a (Glycophorin A) Antibodies
Invitrogen
View all (84) CD235a (Glycophorin A) antibodies
Cite CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), PE-Cyanine7, eBioscience™
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CD235a (Glycophorin A) Antibody (25-9987-42) in Flow
Normal human peripheral blood cells were stained with Mouse IgG2b kappa Isotype Control, PE-Cyanine7 (Product # 25-4732-80) (blue histogram) or CD235a Monoclonal Antibody, PE-Cyanine7 (purple histogram). Cells in the erythrocyte gate were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
25-9987-42
Applications
Tested Dilution
Publications
Flow Cytometry(Flow)
5 µL (0.06 µg)/test
View 17 publications17 publications
Product Specifications
Published species
Not Applicable
Host/Isotype
Mouse/ IgG2b, kappa
Recommended Isotype Control
Mouse IgG2b kappa Isotype Control (eBMG2b), PE-Cyanine7, eBioscience™
Class
Monoclonal
Type
Antibody
Clone
HIR2 (GA-R2)
Conjugate
PE-Cyanine7
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661
- eFluor 450
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- PE
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Unconjugated
Excitation/Emission Max
569/780 nmView spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_2815124
Product Specific Information
Description: The HIR2 monoclonal antibody reacts with human glycophorin A, sialoglycoproteins expressed by erythroid precursors and mature circulating red cells.
Applications Reported: This HIR2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HIR2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Target Information
CD235a (Glycophorins A, GPA) is a single pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. CD235a is the carrier of blood group M and N specificities. CD235a provides cells with a large mucin-like that may serve as a barrier to cell fusion, minimizing aggregation between red blood cells in the circulation. CD235a has been shown to act as a receptor for Sandei virus, parvovirus, and Hsa, an Streptococcus adhesin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases:CD235; CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A (MN blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; Glycophorin-A; HGNC:4702; HGpMiX; HGpStaC; Mi.V glycoprotein; Mi.V glycoprotein (24 AA); MN sialoglycoprotein; PAS-2; recombinant glycophorin A-B Miltenberger-DR; Sialoglycoprotein alpha
Gene Aliases:CD235a; GPA; GPErik; GPSAT; GYPA; HGpMiV; HGpMiXI; HGpSta(C); MN; MNS; PAS-2
UniProt ID:(Human) P02724
Entrez Gene ID:(Human) 2993
Function(s)
viral entry into host cell
Process(es)
virus receptor activityidentical protein binding
It has to be done as per old AB suggested Products section.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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