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- IkB alpha Antibodies
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View all (64) IkB alpha antibodies
Cite Phospho-IkB Alpha (Ser32, Ser36) Recombinant Rabbit Monoclonal Antibody (1G21)
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Phospho-IkB Alpha (Ser32, Ser36) Antibody (82349-1-RR) in WB
Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha-tubulin antibody as loading control. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
82349-1-RR
Applications
Tested Dilution
Publications
Western Blot(WB)
1:5,000-1:50,000
-
ELISA(ELISA)
Assay-dependent
-
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit/ IgG
Expression System
Mammalian cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1G21
Immunogen
Peptide
Conjugate
Unconjugated
Form
Liquid
Purification
Protein A
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
Target Information
IkB-alpha is a 40 kDa protein that functions to inhibit NF- kappaB activity. The inhibition occurs via protein-protein interaction between I kappaB proteins and NF- kappaB dimers in the cytosol. The interaction of I kappa B-alpha with NF- kappaB masks the nuclear localization sequence of NF- kappaB, preventing NF- kappaB translocation to the nucleus. A variety of stimuli can activate gene expression by liberating NF- kappaB through the degradation of I kappaB alpha. These stimuli include the proinflammatory cytokines TNF- alpha and IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, as well as various chemical and physical stresses. In humans, the gene is located on the q arm of chromosome 14. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase alpha (IKK alpha), previously designated CHUK, interacts with IkB-alpha and specifically phosphorylates IkB-alpha on the sites that trigger its degradation Serines 32 and 36. IKK alpha appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKK alpha is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKK alpha, IKK beta and IKK gamma, and each appear to make essential contributions to IkB phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases:I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappa-B inhibitor alpha; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
UniProt ID:(Human) P25963
Entrez Gene ID:(Human) 4792
Function(s)
protein import into nucleus, translocationstimulatory C-type lectin receptor signaling pathwayapoptotic processcytoplasmic sequestering of NF-kappaBnegative regulation of macrophage derived foam cell differentiationpositive regulation of cholesterol effluxnegative regulation of lipid storageviral processlipopolysaccharide-mediated signaling pathwaynegative regulation of NF-kappaB transcription factor activitypositive regulation of cellular protein metabolic processresponse to muramyl dipeptidetoll-like receptor 4 signaling pathwayresponse to muscle stretchFc-epsilon receptor signaling pathwayregulation of cell proliferationregulation of NF-kappaB import into nucleuscytoplasmic sequestering of transcription factornegative regulation of apoptotic processresponse to exogenous dsRNAnegative regulation of DNA bindingnegative regulation of myeloid cell differentiationnegative regulation of Notch signaling pathwaypositive regulation of transcription from RNA polymerase II promoterT cell receptor signaling pathwaypositive regulation of NF-kappaB transcription factor activitycellular response to coldnucleotide-binding oligomerization domain containing 1 signaling pathwaynucleotide-binding oligomerization domain containing 2 signaling pathwaycellular response to cytokine stimuluscellular response to organic cyclic compound
Process(es)
protein bindingtranscription factor bindingnuclear localization sequence bindingenzyme bindingheat shock protein bindingubiquitin protein ligase bindingprotein complex bindingidentical protein bindingNF-kappaB binding
It has to be done as per old AB suggested Products section.
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