Designed specifically for microbial fermentation
The Thermo Scientific HyPerformaSingle-Use Fermentor (S.U.F.) is pioneering the single-use technology market by delivering a unique and rigorous solution for microbial fermentation applications while offering the flexibility, ease of use, and efficiency found in single-use systems.
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The S.U.F. is engineered for optimal oxygen mass transfer, mixing, and temperature control, with performance comparable to conventional stainless-steel systems. S.U.F.s use fundamental engineering principles, helping to make scale-up and process development work readily streamlined. Available in 30 L and 300 L sizes, the S.U.F. consists of a fermentor tank and a standard Thermo Scientific HyPerforma S.U.F. BioProcess Container (BPC).
Benefits of single-use fermentors
HyPerforma Enhanced Single-Use Fermentor Systems (eS.U.F.)
Enhancements to improve performance of single-use fermentors were made to the 300 L hardware, as well as the 30 L and 300 L BPCs, to match more closely the oxygen delivery and cooling capacityperformanceofthestainless steelfermentors.
Hardware enhancements
The cooling jacket surface area was increased on the sides and bottom for the 300 L S.U.F. to support adequate cooling of the production process. This 35% increase jacketed surface area will enable faster cooling of the molecule production process.
Hardware and jacketing surface comparison between HyPerforma S.U.F. (300 L) and HyPerforma eS.U.F. (300 L).
BioProcessing Container (BPC) enhancements
The BPCs for 30 L and 300 L were redesigned to have three parabolic turbine impellers that increase oxygen delivery from spargers by at least four times (4x). As a result, customer and internal culture comparisons showed that oxygen supplementation was reduced 5–7 fold vs. HyPerforma S.U.F., and is comparable to performance of stainless-steel fermentors, per same strain and procedure.
Features of S.U.F and eS.U.F.
Developed with standard stainless-steel fermentation design geometry (3:1 height to diameter, 5:1 turndown ratio), the S.U.F. supports scale-up and process development activities.
- 3:1 total height-to-width aspect ratio for optimal cooling surface to volume ratio
- The BPCs are highly configurable, allowing for flexibility to address specific application needs
- Complete mixing system with a water jacket for temperature control
- Drive shaft inserts into the BPC through the mixing drive motor and locks into the BPC agitator assembly of the S.U.F. BPC
- Integrally sealed ports in the S.U.F. BPC allow for addition of sensor probes and line sets
- Integrated foam sensor
- Single-use pH, DO, temperature, foam, and pressure sensors with clear optical density plug port
- Exhaust management system with options of low or high flowhydrophobicfilter membranes andmultiple vent filters based on gas flow needs
- High flow exhaust condenser with back-up filters, gas sparge filter line sets
- Feed, submerge fill, syringe sample, and drain line set options
Powerful agitation facilitates high oxygen transfer (kLa)and a hom*ogeneous reaction
- 3 Rushton impellers with standard S.U.F. or enhanced parabolic with eS.U.F.
- Impellers couple directly to shaft through bearing to drive motor
- 4 baffles
- Drilled-hole sparge (DHS) with ring-like design
- Innovative exhaust management and condenser systems designed to provide reliable off-gassing and help minimize liquid loss during the fermentation process
System options
- Exhaust condenser unit (extend filter life)
- Exhaust gas vent filter heater(extend filterlife, recommended151 W for 300 L)
- Backup filter automated pinch clamp(extend filter life)
- Load cells
- Cable/ tubingmanagement tree
- Turnkey system withHyPerformaG3 process control system
- Electrical box for remote agitationoropen-architecture control
S.U.F.s require a separate external temperature control unit(45 W/L min. cooling capacity recommendedfor high demanding cultures). You may choose an open-architecture approach or a turnkey, ready-to-use S.U.F. system.
HyPerforma Single-Use Fermentor animation
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Ordering information
Enhanced S.U.F.
S.U.F.
CentriPAK BioProcess Containers
Next-generation centrifuge-ready BioProcess Containers provide a single-use, sterilized, closed system for cell harvesting in bioprocessing.
Resources
- White Paper: Streamlining the upstream workflow for plasmid DNA manufacturing
- Brochure: Single-Use Fermentor
- Data Sheet:30 L Single-Use Fermentor
- Data Sheet: 300 L Single-Use Fermentor
- Application Note: Production of Proteinase K
- Application Note: End-user evaluation of 30 L and 300 L S.U.F. and scale-down model
- Application Note: Production of therapeutic plasmid DNA in single-use fermentors
- Application Note: Thermoinducible expression of DNA polymerase I
- Application Note: Cultivation of aggressive microbial cultures of Pichia and Bacillus using the HyPerforma 30 L and 300 L Single-Use Fermentors
- Application Note:Scale-up of microbial fermentation using recombinant E. coli in HyPerforma 30 L and 300 L Single-Use Fermentors
- Application Note: Production of antibody fragments by S. cerevisiae in the HyPerforma Single-Use Fermentor
- Application Note: Scale-up and tech transfer from 100 L SIP/CIP vessel to 300 L S.U.F.
- Application Note: Mass transfer of single-use fermentors to stirred glass bioreactors
Bioprocessing resources
Access a targeted collection of scientific application notes, case studies, posters, white papers and more for bioprocessing:
- Bioprocessing Learning Center
- Bioprocessing Technical Resource Library
- Download Bioprocessing Product Reference Guide
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