Phospho-AKT (Ser473) Monoclonal Antibody (1C10B8), HRP (HRP-66444) (2024)

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Phospho-AKT (Ser473) Monoclonal Antibody (1C10B8), HRP (HRP-66444) (1)

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  • Primary Antibodies
  • AKT1 Antibodies

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Phospho-AKT (Ser473) Monoclonal Antibody (1C10B8), HRP (HRP-66444) (2)

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Phospho-AKT (Ser473) Antibody (HRP-66444) in WB

Untreated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with HRP-66444 (Phospho-AKT (Ser473) antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more

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Phospho-AKT (Ser473) Monoclonal Antibody (1C10B8), HRP (HRP-66444) (4)

Product Details

HRP-66444

Applications

Tested Dilution

Publications

Western Blot(WB)

1:5,000-1:50,000

-

Product Specifications

Species Reactivity

Human,Mouse

Host/Isotype

Mouse/ IgG1

Class

Monoclonal

Type

Antibody

Clone

1C10B8

Immunogen

Peptide

Conjugate

HRP

  • CoraLite 555
  • CoraLite 594
  • CoraLite Plus 488
  • CoraLite Plus 647
  • Unconjugated

Form

Liquid

Purification

Protein A

Storage buffer

PBS with 0.5% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, store in dark

Shipping conditions

Wet ice

Target Information

AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases:AKT1 kinase; AKT1m; Akt1m protein; C-AKT; CAKT; PKB; PKB alpha; PKBG; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; related to A and C kinases; serine-threonine protein kinase; Thymoma viral proto-oncogene; v-akt murine thymoma viral oncogene hom*olog 1; v-akt murine thymoma viral oncogene-like protein 1

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Gene Aliases:AKT; AKT1; CWS6; PKB; PKB-ALPHA; PKB/Akt; PKBalpha; PRKBA; RAC; RAC-ALPHA

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Molecular Function: Phospho-AKT (Ser473) Monoclonal Antibody (1C10B8), HRP (HRP-66444) (5)non-receptor serine/threonine protein kinase

Function(s)

protein import into nucleus, translocationosteoblast differentiationmaternal placenta developmentpositive regulation of protein phosphorylationpositive regulation of endothelial cell proliferationglycogen biosynthetic processregulation of glycogen biosynthetic processglucose metabolic processtranslationregulation of translationcellular protein modification processprotein phosphorylationnegative regulation of protein kinase activitynitric oxide biosynthetic processactivation-induced cell death of T cellsinflammatory responsecellular response to DNA damage stimulusresponse to oxidative stresssignal transductionG-protein coupled receptor signaling pathwaygerm cell developmentagingcell proliferationinsulin receptor signaling pathwayapoptotic mitochondrial changesresponse to heatnegative regulation of autophagynegative regulation of gene expressionnegative regulation of plasma membrane long-chain fatty acid transportpositive regulation of fibroblast migrationpositive regulation of sodium ion transportpositive regulation of glucose metabolic processnegative regulation of endopeptidase activityregulation of neuron projection developmentregulation of phosphatidylinositol 3-kinase signalingglucose transportphosphorylationprotein ubiquitinationpeptidyl-serine phosphorylationpeptidyl-threonine phosphorylationspinal cord developmentcell projection organizationcell differentiationprotein catabolic processplatelet activationhyaluronan metabolic processpositive regulation of cell growthregulation of cell migrationendocrine pancreas developmentT cell costimulationregulation of myelinationpositive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cyclelipopolysaccharide-mediated signaling pathwaynegative regulation of fatty acid beta-oxidationpositive regulation of endodeoxyribonuclease activityresponse to foodpositive regulation of cellular protein metabolic processperipheral nervous system myelin maintenancepositive regulation of proteasomal ubiquitin-dependent protein catabolic processcellular response to insulin stimuluspositive regulation of peptidyl-serine phosphorylationresponse to fluid shear stressintracellular signal transductioncellular response to vascular endothelial growth factor stimulusERBB2 signaling pathwayglucose homeostasispositive regulation of apoptotic processnegative regulation of apoptotic processnegative regulation of cysteine-type endopeptidase activity involved in apoptotic processregulation of mRNA stabilityprotein kinase B signalingpositive regulation of blood vessel endothelial cell migrationpositive regulation of nitric oxide biosynthetic processpositive regulation of fat cell differentiationpositive regulation of glycogen biosynthetic processpositive regulation of epidermal growth factor receptor signaling pathwaynegative regulation of cell sizenegative regulation of proteolysispositive regulation of vasoconstrictionpositive regulation of transcription from RNA polymerase II promoterpositive regulation of glucose importnegative regulation of JNK cascadeprotein autophosphorylationpositive regulation of lipid biosynthetic processinsulin-like growth factor receptor signaling pathwayphosphatidylinositol-mediated signalingregulation of nitric-oxide synthase activitypositive regulation of nitric-oxide synthase activitypositive regulation of sequence-specific DNA binding transcription factor activitystriated muscle cell differentiationresponse to growth hormonemammary gland epithelial cell differentiationglycogen cell differentiation involved in embryonic placenta developmentlabyrinthine layer blood vessel developmentresponse to UV-Acellular response to mechanical stimuluscellular response to epidermal growth factor stimuluscellular response to prostaglandin E stimuluscellular response to organic cyclic compoundcellular response to hypoxiaestablishment of protein localization to mitochondrionmaintenance of protein location in mitochondrionpositive regulation of establishment of protein localization to plasma membranenegative regulation of release of cytochrome c from mitochondriacellular response to granulocyte macrophage colony-stimulating factor stimulusexecution phase of apoptosischemical synaptic transmission, postsynapticnegative regulation of protein kinase activity by protein phosphorylationpositive regulation of protein localization to nucleusnegative regulation of neuron deathregulation of signal transduction by p53 class mediatorregulation of cell cycle checkpointnegative regulation of oxidative stress-induced intrinsic apoptotic signaling pathwaycellular response to nerve growth factor stimulusresponse to insulin-like growth factor stimulusnegative regulation of extrinsic apoptotic signaling pathway in absence of ligandcarbohydrate metabolic processglycogen metabolic processtransportapoptotic processcytoskeleton organizationmulticellular organism developmentnervous system developmentcarbohydrate transportresponse to hormoneregulation of protein localizationcellular response to decreased oxygen levelsanagencellular response to growth factor stimuluscellular response to peptide

Process(es)

protein kinase activityprotein serine/threonine kinase activityprotein serine/threonine/tyrosine kinase activityprotein kinase C bindingprotein bindingATP bindingphosphatidylinositol-3,4,5-trisphosphate bindingkinase activityenzyme bindingnitric-oxide synthase regulator activityGTPase activating protein bindingidentical protein bindingphosphatidylinositol-3,4-bisphosphate bindingprotein phosphatase 2A binding14-3-3 protein bindingnucleotide bindingtransferase activitytransferase activity, transferring phosphorus-containing groupsprotein kinase binding

It has to be done as per old AB suggested Products section.

Phospho-AKT (Ser473) Monoclonal Antibody (1C10B8), HRP (HRP-66444) (6)

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Phospho-AKT (Ser473) Monoclonal Antibody (1C10B8), HRP (HRP-66444) (7)

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Phospho-AKT (Ser473) Monoclonal Antibody (1C10B8), HRP (HRP-66444) (2024)
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