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- PD-1 (CD279) Antibodies
Invitrogen
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
View all (262) PD-1 (CD279) antibodies
Cite CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), PE-Cyanine7, eBioscience™
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CD279 (PD-1) Antibody (25-2799-42) in Flow
Staining of 3-day PHA-stimulated normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD279 (PD-1) PE-Cyanine7 (purple histogram). Cells in the lymphocyte gate were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
25-2799-42
Applications
Tested Dilution
Publications
Flow Cytometry(Flow)
5 µL (0.5 µg)/test
View 15 publications15 publications
Product Specifications
Species Reactivity
Human,Rhesus monkey
Published species
Human,Rhesus monkey
Recommended Isotype Control
Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™
Class
Monoclonal
Type
Antibody
Clone
eBioJ105 (J105)
Conjugate
PE-Cyanine7
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 615
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 650
- Brilliant Violet 711
- Brilliant Violet 786
- PE
- PE-Cyanine5
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Unconjugated
Excitation/Emission Max
569/780 nmView spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_10853804
Product Specific Information
Description: The J105 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells.
Costaining experiments suggest that eBioJ105 recognizes a different epitope than MIH4 (Product # 11-9969).
Applications Reported: This eBioJ105 (J105) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioJ105 (J105) antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases:CD279; hPD1; programmed cell death 1 protein; Programmed cell death protein 1; Protein PD-1; Protein PD1; sCD279; soluble CD279; systemic lupus erythematosus susceptibility 2
UniProt ID:(Human) Q15116
Entrez Gene ID:(Human) 5133, (Rhesus monkey) 100135775
Function(s)
negative regulation of tolerance inductionapoptotic processhumoral immune responsesignal transductionmulticellular organism developmentT cell costimulationnegative regulation of apoptotic processpositive regulation of T cell apoptotic process
Process(es)
signal transducer activityprotein binding
It has to be done as per old AB suggested Products section.
