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- Primary Antibodies ›
- CD235a (Glycophorin A) Antibodies
Invitrogen
View all (84) CD235a (Glycophorin A) antibodies
Cite CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), eFluor™ 450, eBioscience™
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CD235a (Glycophorin A) Antibody (48-9987-42) in Flow
Staining of normal human peripheral blood cells with Mouse IgG2b K Isotype Control eFluor® 450 (Product # 48-4732-82) (blue histogram) or Anti-Human CD235a (Glycophorin A) eFluor® 450 (purple histogram). All non-leukocytes were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
48-9987-42
Applications
Tested Dilution
Publications
Immunocytochemistry(ICC/IF)
-
View 1 publication1 publication
Flow Cytometry(Flow)
5 µL (0.03 µg)/test
View 28 publications28 publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse/ IgG2b, kappa
Recommended Isotype Control
Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 450, eBioscience™
Class
Monoclonal
Type
Antibody
Clone
HIR2 (GA-R2)
Conjugate
eFluor™ 450
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Unconjugated
Excitation/Emission Max
405/445 nmView spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_2574141
Product Specific Information
Description: The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells.
Applications Reported: This HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HIR2 (also GA-R2) antibody has been pre-titrated and tested by flow cytometric analysis of diluted normal human peripheral blood cells. Binding of this antibody to red cells at high antibody concentration causes cell agglutination. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD235a (Glycophorins A, GPA) is a single pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. CD235a is the carrier of blood group M and N specificities. CD235a provides cells with a large mucin-like that may serve as a barrier to cell fusion, minimizing aggregation between red blood cells in the circulation. CD235a has been shown to act as a receptor for Sandei virus, parvovirus, and Hsa, an Streptococcus adhesin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases:CD235; CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A (MN blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; Glycophorin-A; HGNC:4702; HGpMiX; HGpStaC; Mi.V glycoprotein; Mi.V glycoprotein (24 AA); MN sialoglycoprotein; PAS-2; recombinant glycophorin A-B Miltenberger-DR; Sialoglycoprotein alpha
Gene Aliases:CD235a; GPA; GPErik; GPSAT; GYPA; HGpMiV; HGpMiXI; HGpSta(C); MN; MNS; PAS-2
UniProt ID:(Human) P02724
Entrez Gene ID:(Human) 2993
Function(s)
viral entry into host cell
Process(es)
virus receptor activityidentical protein binding
It has to be done as per old AB suggested Products section.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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