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- CD154 (CD40 Ligand) Antibodies
Invitrogen
View all (82) CD154 (CD40 Ligand) antibodies
Cite CD154 (CD40 Ligand) Monoclonal Antibody (MR1), Functional Grade, eBioscience™
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CD154 (CD40 Ligand) Antibody (16-1541-82) in Flow
Staining of 6-hour, PMA/Ionomycin-stimulated T cells from BALB/c spleen with 0.25 µg of Armenian Hamster IgG Isotype Control Functional Grade Purified (Product # 16-4888-81) (open histogram) or 0.25 µg of Anti-Mouse CD154 Functional Grade Purified (right) followed by Anti-Armenian Hamster IgG Biotin (Product # 13-4113-85) and Streptavidin PE (Product # 12-4317-87).Total viable cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
16-1541-82
Applications
Tested Dilution
Publications
Flow Cytometry(Flow)
0.5 µg/test
View 11 publications11 publications
Neutralization(Neu)
Assay-Dependent
View 1 publication1 publication
Functional Assay(FN)
Assay-Dependent
View 2 publications2 publications
Inhibition Assays(IA)
-
View 1 publication1 publication
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Armenian hamster/ IgG
Recommended Isotype Control
Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™
Class
Monoclonal
Type
Antibody
Clone
MR1
Conjugate
Functional Grade
- Alexa Fluor 488
- APC
- Biotin
- eFluor 450
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Wet ice
RRID
AB_469085
Product Specific Information
Description: The MR1 monoclonal antibody reacts with mouse CD154, a 39 kDa transmembrane glycoprotein also known as CD40 ligand (CD40L). CD154 is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells (APC) including B cells, monocytes/macrophages, and dendritic cells, it serves a crucial function in T cell-APC cognate interaction. CD154-interaction with CD40 transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle.
For staining for flow cytometric analysis, it is important to stimulate enriched T cells or enriched CD4 cells (using depletion strategy) prior to staining with MR1.
Applications Reported: The MR1 antibody has been reported for use in flow cytometric analysis. It has also been reported to inhibit binding of CD154 to CD40 and activation of B cells.
Applications Tested: This MR1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases:CD154; CD40 ligand; CD40-L; H-CD-40-L; M-CD-40-L; RP23-153G22.3; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5
Gene Aliases:CD154; CD40-L; Cd40l; Cd40lg; gp39; HIGM1; IGM; IMD3; Ly-62; Ly62; T-BAM; Tnfsf5; TRAP
UniProt ID:(Mouse) P27548
Entrez Gene ID:(Mouse) 21947
Function(s)
positive regulation of immune response to tumor cellinflammatory responseimmune responsesignal transductionplatelet activationB cell differentiationpositive regulation of B cell proliferationpositive regulation of interferon-gamma productionpositive regulation of interleukin-10 productionpositive regulation of interleukin-12 productionB cell proliferationnegative regulation of apoptotic processisotype switchingpositive regulation of MHC class II biosynthetic processimmunoglobulin secretionregulation of immunoglobulin secretiondendritic cell differentiationpositive regulation of endothelial cell apoptotic process
Process(es)
cytokine activitytumor necrosis factor receptor bindingCD40 receptor binding
It has to be done as per old AB suggested Products section.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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