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- ATG5 Antibodies
Invitrogen
View all (54) ATG5 antibodies
Cite ATG5 Polyclonal Antibody
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ATG5 Antibody (PA5-29694) in ICC/IF
ATG5 Polyclonal Antibody detects ATG5 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50 µM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG5 protein stained by ATG5 Polyclonal Antibody (Product # PA5-29694) diluted at 1:1,000. Red: Phalloidin, a F-actin marker. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
PA5-29694
Applications
Tested Dilution
Publications
Western Blot(WB)
1:500-1:3,000
-
Immunohistochemistry (Paraffin) (IHC (P))
1:100-1:1,000
-
Immunocytochemistry(ICC/IF)
1:100-1:1,000
-
in situ PLA(PLA)
Assay-dependent
-
Product Specifications
Species Reactivity
Human,Mouse
Host/Isotype
Rabbit/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 64 and 275 of Human APG5
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547168
Product Specific Information
Recommended positive controls: NT2D1, PC-3, HeLa, HeLa (chloroquine treatment for 24hr).
Predicted reactivity: Mouse (95%), Xenopus laevis (81%), Chicken (87%), Bovine (95%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATG5 (Autophagy Related 5) is an important element for autophagy and may play an important role in the apoptotic process. ATG5 is also involved in other cellular processes that include mitochondrial quality control after oxidative damage, negative regulation of the innate anti-viral immune response, lymphocyte development and proliferation, MHC II antigen presentation, and adipocyte differentiation. Following conjugation to ATG12, the conjugate participates in the formation of autophagosome. ATG5 contributes to autophagic cell death by interacting with Fas-associated protein with death domain (FADD). The ATG5-ATG12 conjugate forms a cup-shaped isolation membrane that then detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may play a role in the apoptotic process, possibly within the modified cytoskeleton. Further, APG5 expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. The APG5-APG12 conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. Diseases associated with ATG5 dysfunction include spinocerebellar ataxia.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases:APG 5; APG5 autophagy 5 like (APG5L); APG5-like; Apoptosis-specific protein; Apoptosis-specific protein (ASP); ATG5 autophagy related 5 hom*olog; Autophagy protein 5; autophagy-related 5
Gene Aliases:2010107M05Rik; 3110067M24Rik; APG5; APG5-LIKE; APG5L; ASP; ATG5; Atg5l; AW319544; C88337; hAPG5; Paddy
UniProt ID:(Human) Q9H1Y0, (Mouse) Q99J83
Entrez Gene ID:(Human) 9474, (Mouse) 11793
Molecular Function: 
membrane trafficking regulatory protein
Function(s)
autophagosome assemblymitophagyblood vessel remodelingregulation of cytokine secretion involved in immune responseC-terminal protein lipidationautophagyapoptotic processcellular response to nitrogen starvationresponse to fungusmacroautophagycellular homeostasisantigen processing and presentation of endogenous antigennegative regulation of protein ubiquitinationaggrephagynegative stranded viral RNA replicationvasodilationresponse to drugnegative regulation of apoptotic processpost-translational protein modificationnucleophagynegative thymic T cell selectioninnate immune responseotolith developmentregulation of release of sequestered calcium ion into cytosolventricular cardiac muscle cell developmentheart contractionnegative regulation of cell deathprotein lipidation involved in autophagosome assemblypositive regulation of mucus secretionregulation of cilium assemblypositive regulation of mitophagynegative regulation of reactive oxygen species metabolic processnegative regulation of histone H4-K16 acetylationimmune system processregulation of reactive oxygen species metabolic process
Process(es)
protein bindingAtg8 ligase activity
It has to be done as per old AB suggested Products section.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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